Azospirillum strains, Acetobacter and Azotobacter sp. were isolated from roots of prickly pear, tomato and pepper, and characterized according to nitrogenase activity and indoleacetic acid production. The best strains were selected to evaluate their effect on seed germination and vigor in jalapeño pepper (Capsicum annuum L. 'var. Grande'). Inoculation of seed was performed with different inoculum concentration (104, 106 and 108 CFU mL-1) of each strain. A completely randomized design with two factors:A) strains and B) inoculum concentration, was used. AzTCH3 strain isolated from the rhizosphere of pepper in Torreon, promoted 7% seed germination. Fresh biomass was stimulated 20% by Azotobacter sp. AzoGN. Dry biomass was favored by AzTT2 Azospirillum sp. inoculating dose of 108 CFU mL-1. Root elongation was increased 32% with Acetobacter sp. AceTT isolated the tomato of Torreon. According to the characterization, of the strains isolated from the rhizosphere of prickly pear (AceGN2), Acetobacter sp. showed the highest production of indoleacetic acid, causing no significant effect on plant development. So, a correlation between variables, it was positive and it is concluded that native rhizobacteria have potential to stimulate plant growth and seed development in jalapeño pepper was due to its production capabilities indoleacetic acid and nitrogenase activity.