M. Martínez-Giner, R.N. Pena, J. Casellas, I. Balcells, A. Fernández, J.L. Noguera
Issue:
104-2 (89-93)
Topic:
Animal Production
Keywords:
Vinculin, Immunohistochemistry, Western blot, Microarrays
Summary:
The objective of this experiment is to study genes and proteins differing across reproductive stages in
swine. RNA from ovary, from sows on heat, 15 and 45 days of pregnancy have been hybridised in porcine
oligonucleotide microchips to characterize changes in gene expression profile between different
reproductive stages. Expression differences in 281 genes (posterior probability <10-11) have been found
between expression at different stages in ovary. One of these genes, vinculin, showed 100 times more
expression on heat than at 45 days of pregnancy, so we chose that gene for immunohistochemistry and
western blot analysis. On immunohistochemistry we found that ovaries of sows on heat showed stronger
vinculin staining than ovaries stroma of sows at 30 days of pregnancy. On western blot, significant
differences appeared only between heat and 45 days of pregnancy.
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