|
|
Adjacent mutations to ovine PrnP codons 136 and 171 affect performance of the diagnostic protocol based on RT-PCR coupled to fluorescence probes |
|
Authors: | A. Traoré, L.J. Royo, I. Álvarez, I. Fernández, J.P. Gutiérrez, C. Rincón, L. Pérez-Pardal, F. Goyache | Issue: | 104-2 (106-109) | Topic: | Animal Production | Keywords: | Sheeps, Scrapie, Subsaharian Africa, RT_PCR, West África | Summary: | In this work we show the problems we have found, in the performance of a diagnostic protocol for
the 136, 154 and 171 codons of the PrnP ovine gene based on RT-PCR, when mutations exist in the
adjacent nucleotides of these positions. A total of 90 individuals belonging to 3 Spanish (Xalda,
Rubia del Molar and Colmenareña) and 3 Burkina Faso sheep breeds (Sahel, Djallonké and Mossi)
were genotyped. Only 3 alleles (ARQ, ARR y AHQ) were found in the Burkinabé breeds. Diagnostic
could not be carried out on codons 136 and 171 in 3 Burkinabé individuals. A total of 410 base pairs
of the PrnP gene including codons 136, 154 and 171 were sequenced allowing the identification of
polymorphisms on codons 138, 143 and 172. The identified polymorphisms affected diagnostic
performance. Programmes using the PrnP genotype as selection criterion could also be affected. |
| Back to Issue 104-2 |
|
|